20. März 2015

Neue Metabolomik-AppNote: Glycoside mittels LC/MS

Die voll-automatisierte Probenvorbereitung umfasst eine Ultraschall-Extraktion von Pflanzenmaterial und eine zweistufige Filtration für die LC-QTOF-Analyse der Glycoside


Automated sample preparation was developed for the extraction of glycosides from plant material using the Gerstel MPS Dual Head WorkStation. Ultrasonic extraction was performed on the plant material, and the extract was prepared for subsequent analysis by two filtration steps. To prevent blockage of the 1.0 mL syringe used for extract transfer, individual 17 μm stainless steel screen filters placed in the sample vials were utilized. Due to the nature of the sample, an additional filtration step using a 0.45 μm syringe filter was required before injection to the LC-MS instrument. Excellent reproducibility was obtained.


Automated Ultrasonic Extraction of a Plant Material Using the Gerstel MPS Dual Head WorkStation